Effect of the Hydrogen Ion Concentration on the Phagocytosis and Adhesiveness of Leucocytes
نویسنده
چکیده
In a previous paper (1) a method has been described for measuring the adhesiveness of leucocytes to glass surfaces, and a series of results obtained by this method was reported. As a further calibration of the method it was necessary to determine what variations in adhesiveness could be produced by varying the H ion concentration. Since the method was originally devised as a means of comparing the spreading of leucocytes on flat surfaces (adhesiveness) with their spreading on surfaces of small solid particles (phagocytosis), the effect of variations in pH on phagocytosis of solid particles has also been investigated. These two series of experiments will be reported together. This subject has been investigated qualitatively by Sawtchenko and Aristowsky (2) for leucocytes, and they found a maximum number of leucocytes sticking to their test-tubes in neutral or slightly alkaline solutions. Loeb (3) reports collaborative experiments by Blanchard indicating an optimum acidity for the migration of amebocytes of Limulus. In more alkaline solutions they adhere to the glass and disintegrate, while in more acid solutions their consistency is so great that they cannot move. For a further investigation of this subject measurements of the adhesiveness of leucocytes to glass at varying DH were made by the stream and gravity methods already described. In the former method the leucocytes are allowed to settle on a glass slide forming the bottom of a glass vessel When the vessel is rotated about a horizontal axis the resulting circulating stream of solution washes off a certain percentage of the leucocytes on the bottom. This percentage is determined by hemocytometer counts. In the gravity method a 169
منابع مشابه
Effect of the Hydrogen Ion Concentration on the Phagocytosis and Adhesiveness of Leucocytes
1. Immediately after coming into contact with glass, leucocytes are most adhesive at pH 8.0 or > 8.0. 2. Agglutination of leucocytes increases with increasing H ion concentration from pH 8.0 to 6.0. 3. In phagocytosis experiments where leucocytes creep about on the slide picking up articles the optimum pH is 7.0. Here ameboid movement is probably the limiting factor. 4. The optimum for phagocyt...
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